Biotinylation is a key technology in modern biology, allowing the specific labeling of proteins, antibodies, nucleic acids, and other molecules with biotin. This label binds with high affinity and specificity to avidin, streptavidin, or neutravidin, forming the basis for numerous applications:

· Immunodetection: Signal amplification in ELISA, Western blot, immunohistochemistry (IHC), and flow cytometry (FACS).

· Affinity Purification: Isolation of specific targets (e.g., biotinylated DNA/RNA, proteins) from complex mixtures using avidin-containing resins.

· Immobilization: Fixation of biotinylated molecules onto (strept)avidin-coated surfaces for biosensors or microarrays.

· Interaction Studies: Investigation of protein-protein or protein-nucleic acid interactions.

Advantages of Lumiprobe Biotinylation Reagents:

· High Purity & Stability: Guaranteed quality for reproducible results.

· Broad Selection: Solutions for virtually any labeling strategy and target molecule type.

· Optimized Spacers: PEG spacers minimize steric hindrance, enhancing biotin tag accessibility for (strept)avidin binding.

· Competitive Pricing: Cost-effective solutions without compromising quality.

· Biotin-PEG4-NHS ester

· Biotin-XX-NHS ester

· Biotin-X-maleimide

· Biotin-X-hydrazide

· Biotin-PEG2-alkyne

· Biotin-PEG4-azide