[CytoTest 공식대리점 관련 업무대행/수입] Fluorescence in situ Hybridization (FISH)

어스바이오는 CytoTest 공식대리점 관련 업무대행/수입 전문취급점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다. 

FISH has become a central cytogenetic tool. Probe fragments can be derived from various sources, by recombinant methods or by PCR. Today, accurately mapped bacterial artificial chromosomes (BAC) are the most frequently used probe type. Designing or selecting the right probe for FISH often requires a balance between sensitivity and specificity, i.e. a probe must be large enough to assure specific binding and to carry a sufficient amount of label for detection. On the other hand, with increasing length of the probe the possibility of hybridization to additional target sequences increases as well. In addition, if a probe is too long it may result in a large or diffuse signal.

With FISH, chromosomal rearrangements or aberrations of as small as a few megabase pairs (million base pairs) can be made visible on metaphase (condensed) chromosomes. The resolution goes down to about 1Mb for interphase (spread out) chromosomes. Even greater resolution can be achieved with recently developed methods encompassing artificial stretching of chromatin fibers.

FISH methods cannot detect base-level mutations, e.g. the point mutations underlying some heritable disorders. However, FISH techniques have made landmark contributions in moving the genetic and physical maps of the human genome closer together.