[Sylphium molecular ecology 공식대리점 관련 업무대행/수입] eDNA qPCR hot start mix 제품소개

• Description:

The eDNA qPCR hot start mix has:

· Highest resistance to inhibiting factors in comparison to other qPCR master mixes. Environmental DNA extracts contain often multiple qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.

· Highest fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto fluoresce substances that will interfere with the measurements. A strong fluorescence signal from the analyses is required for this kind of samples.

· Highest possible sensitivity (1 DNA copy per reaction). Environmental water samples may contain very low amounts of target DNA.

· Hot-start properties. The DNA polymerase enzyme has been genetically modified to have similar or better hot-start properties when compared to antibody-mediated hot-start enzymes. Working on ice is not necessary during the preparation of the PCR mixture.

Relevant Documents:

· Protocol eDNA qPCR hot start mix (SYL1003)

· Validation report eDNA qPCR hot start mix (SYL1003)

· Lot validation documents: 220114, 220611, 221114

· Material safety data sheets: Sylphium eTaq qPCR mix, Sylphium eTaq DNA polymerase

References:

Uchii, K., Doi, H., Okahashi, T., Katano, I., Yamanaka, H., Sakata, M. K., et al. (2019). Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA. DNA 1, 359–367.